biology work

1. Use the graph above to calculate the questions below. Note that values for the time points are listed next to the data point. Carry math out to 4th decimal place

a. Growth rate and doubling time using OD values from time points 120min and 360min

b. Growth rate and doubling time using viable cells/ml values from time points 360min and 480min.

c. Cell death rate using viable cells/ml values from time points 540min and 600min.

d. Why does the OD value stay high while the viable cell count drops drastically at 540 – 600min? How does this reflect the importance of using viable cell count over OD values when determining growth rate?

2. What are the advantages of batch operation? What are the advantages of continuous operation? With the disadvantages of both operational modes in mind which would be best for CHO cells? Which method would be best for E. coli? Why?

Cell type: Operation Method: Reasoning:
CHO
E.coli

3. How does the Trypan Blue Dye Exclusion assay work? How would this be useful in culture sampling?

4. What is the surface area of a bubble with a volume of 5mL? What is the total surface area of 10 bubbles with a volume of 0.5mL each? (1mL = 1cm3)

5. The air sparge of the bioreactor chamber produces bubbles with a volume of 0.10mL. The maximum number of bubbles that can be produced at any one time is 1000. The minimum number of bubbles that can be produced is 200. What is the range of surface area available for aeration?

6. What is shear damage? What cell types are most prone to shear damage? What bioreactor types are more prone to cause shear damage?

7. Can air bubbles damage cells? How?

8. What is cell passaging? What is senescence? How does cell passaging help avoid this?

9. What is the largest limiting factor for anchored cell types? What is a means that researchers use to compensate for this factor?

10. What is the ambr bioreactor? How is growth controlled? How could this bioreactor help with research or product development?

11. What are the factors of cell culture considerations? Which factor do you feel would be the most important consideration for bioproduction? Why? Which factor do you feel would be the most important consideration for research? Why?

12. Select 4 bioreactors from the list below. For each bioreactor selected, select which type of media applies. Give an example of the specific type of cells/organisms grown in the bioreactor with the specific media type. If bioreactor uses both media/cell types; give an example of each:

Bioreactors: Petri dish, Tissue Flask, Baffled flask, Roller Bottle, spinner flask, biofermentor, microcarrier, Hollow Fiber system, Cell factory, Wave Bioreactor, Bellocell

For media type either: Box, Underline or Bold selected answer

Bioreactor Type: Media Type
Suspension Anchored Both
Example: Cell/Organism:
Growth Media:

Bioreactor Type: Media Type
Suspension Anchored Both
Example: Cell/Organism:
Growth Media:

Bioreactor Type: Media Type
Suspension Anchored Both
Example: Cell/Organism:
Growth Media:

Bioreactor Type: Media Type
Suspension Anchored Both
Example: Cell/Organism:
Growth Media:

E.coli Sample Set – Growth Rate

OD 0.0 60.0 120.0 180.0 240.0 300.0 360.0 420.0 480.0 540.0 600.0 0.021 0.038 0.095 0.342 0.711 1.139 1.356 1.472 1.55 1.554 1.564 Viable Cells 0.0 60.0 120.0 180.0 240.0 300.0 360.0 420.0 480.0 540.0 600.0 0.0 0.0 0.0 0.0800000000000001 0.09 0.1 0.44 1.8 4.7 4.9 1.05

Time (min)

OD 600nm

Viable cells/mL x107